Donor Islet Endothelial Cells in Pancreatic Islet Revascularization
نویسندگان
چکیده
منابع مشابه
Donor Islet Endothelial Cells in Pancreatic Islet Revascularization
OBJECTIVE Freshly isolated pancreatic islets contain, in contrast to cultured islets, intraislet endothelial cells (ECs), which can contribute to the formation of functional blood vessels after transplantation. We have characterized how donor islet endothelial cells (DIECs) may contribute to the revascularization rate, vascular density, and endocrine graft function after transplantation of fres...
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Pancreatic islet transplantation has emerged as a therapy for type 1 diabetes and is today performed using both freshly isolated and cultured islets. Islet blood vessels are disrupted during islet isolation; therefore, proper revascularization of the transplanted islets is of great importance for islet graft function and survival. We have studied intraislet endothelial cells after islet isolati...
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To investigate molecular mechanisms controlling islet vascularization and revascularization after transplantation, we examined pancreatic expression of three families of angiogenic factors and their receptors in differentiating endocrine cells and adult islets. Using intravital lectin labeling, we demonstrated that development of islet microvasculature and establishment of islet blood flow occu...
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A system of digitonin-permeabilized islet cells was developed to characterize Ca2+- and calmodulin-dependent protein phosphorylation further and to determine whether activation of this membrane-bound process was sufficient for initiation of Ca2+-stimulated insulin secretion. The efficacy of digitonin in permeabilizing the plasma membrane was assessed by Trypan Blue exclusion, by extracellular l...
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In the course of ultrastructural studies on the morphology of the pancreatic islets of various rodent species used in current diabetes research, intranuclear rod-shaped structures were observed in the $-cells of mice . Since there appeared to be no previous reports on the occurrence of such structures in endocrine pancreas of any species, it was thought worth while to pay some further attention...
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ژورنال
عنوان ژورنال: Yearbook of Endocrinology
سال: 2012
ISSN: 0084-3741
DOI: 10.1016/j.yend.2012.03.056